| Current Zoology(formerly Acta Zoologica Sinica), 2010, 56(2): 252 - 258 |
|
| Title: | Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation |
| Authors: | Jing SUN, Shuiyan YU, Zhuang XUE, Cenjie LIU, Yu WU, Xin LIU, Qingwei LI |
| I n s t i t u t e o f M a r i n e G e n o m i c s & P r o t e o m i c s , L i a o n i n g N o r m a l U n i v e r s i t y , D a l i a n 1 1 6 0 2 9 , C h i n a |
|
| Abstract: | Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete anticoagulant from their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2 (BGSP-2) from a buccal gland cDNA library of Lampetra japonica. The full-length BGSP-2 gene was cloned and the recombinant BGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects on human T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by inducing G1/S cell cycle arrest. Furthermore, it inhibited the proliferation of human T lymphocytes stimulated by phytohemagglutinin (PHA) at a minimum concentration of 0.1μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of human T lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T lymphocytes [Current Zoology 56 (2): 252–258, 2010]. |
| Keywords: | Lampetra japonica, BGSP-2, Cell cycle, T lymphocyte, Proliferation |
*Correspondence should be addressed to Qingwei LI (E-mail:liqw@263.net). |
|
此页被浏览530次，全文被下载29次。
